NEFL 抗体
Quick Overview for NEFL 抗体 (ABIN114492)
抗原
See all NEFL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- 2F11 reacts exclusively with the phosphorylated isoform of the 70 kDa neurofilament protein. This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.
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交叉反应 （详细）
- Also reacts to: Neurofilament H
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纯化方法
- Purified
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免疫原
- Human neurofilament preparation
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亚型
- IgG1
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anti-Neurofilament, Light Polypeptide (NEFL) antibody
NEFL 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 400-543) antibodyNEFL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人, 大鼠, Pig, Cow, 小鸡 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-360) antibodyNEFL 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3G2 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-284) antibodyNEFL 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 128-271) antibodyNEFL 适用: 人 IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on frozen and paraffin embedded sections: This antibody may bediluted to a titer of 1: 50-1: 200 in an ABC method. Immunoblotting: 1: 200 -1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
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- NEFL (Neurofilament, Light Polypeptide (NEFL))
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别名
- Neurofilament L (68kDa)
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背景
- Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiationSynonyms: 68 kDa neurofilament protein, NEFL, NF-L, NF68, Neurofilament L
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分子量
- 68 kDa and 208 kDa (phosphorylated forms of these subunits).
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基因ID
- 4747
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NCBI登录号
- NP_006149
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UniProt
- P07196
抗原
-
