Cytokeratin 7 抗体
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- 抗原 See all Cytokeratin 7 (KRT7) 抗体
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytokeratin 7 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody reacts exlcusively with Cytokeratin 7 which is present in specific glandular-type epithelia and most carcinomas derived thereof.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Purified
- 免疫原
- A cytoskeletal preparation of OTN- 1 ovarian carcinoma cell line
- 克隆位点
- OVTL-12-30
- 亚型
- IgG1
- Top Product
- Discover our top product KRT7 Primary Antibody
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anti-Keratin 7 (KRT7) (AA 251-350) antibody
KRT7 适用: 人, 小鼠 WB, FACS, IHC (p), ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 91-394) antibodyKRT7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 394-457) antibodyKRT7 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal OV-TL12-30 unconjugated
anti-Keratin 7 (KRT7) (AA 1-94) antibodyKRT7 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 2-469) antibodyKRT7 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal rOV-TL12-30 unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal 7D8G8 unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT7-760-OV-TL12-30 unconjugated
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- 应用备注
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Immunohistochemistry on frozen and paraffin embedded sections (1: 100 - 1: 200 with anABC method). Antibody ABIN114453 is effective on frozen tissue after fixation in methanol. It is particularly effective on paraffin-embedded tissue, for which pretreatment with e. g.0.1 % pronase / 0.1 % trypsine in PBS or 0.5 % pepsine in 0.01N HCl or microwavepretreatment is required. Also suitable for immunocytochemistry, immunoblotting (1: 100 - 1: 1000) and flowcytometry (1: 100 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS containing 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the product undiluted Prior to reconstitution at 2-8 °C and after reconstitution (in aliquots) at -20 °C.
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- 抗原
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
- 别名
- Cytokeratin 7 (KRT7 产品)
- 别名
- CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody
- 背景
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
- 基因ID
- 3855
- NCBI登录号
- NP_005547
- UniProt
- P08729
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