Keratin 10 抗体
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- 抗原 See all Keratin 10 (KRT10) 抗体
- Keratin 10 (KRT10)
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适用
- 人, 犬, Cat
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 10 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- DE-K10 reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
- 纯化方法
- Purified
- 免疫原
- Cytoskeletal preparation extracted from human epidermis.
- 克隆位点
- DE-K10
- 亚型
- IgG1
- Top Product
- Discover our top product KRT10 Primary Antibody
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- 应用备注
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Immunoblotting (1/100-1/1000). Immunocytochemistry. Immunohistochemistry on frozen sections and paraffin-embedded tissues (1/100-1/200 forImmunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent). Flow cytometry (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store lyophilized antibody at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for up to two weeks. For longer storage aliquot and store frozen at -20 °C to -80 °C.
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- 抗原
- Keratin 10 (KRT10)
- 别名
- Cytokeratin 10 (KRT10 产品)
- 背景
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK-10, CK10, Cytokeratin-10, K10, KPP, KRT10, Keratin, Keratin-10, Type I Cytoskeletal 10
- 基因ID
- 3858
- NCBI登录号
- NP_000412
- UniProt
- P13645
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