MHC Class II I-Abd 抗体
The 小鼠 单克隆 anti-MHC Class II I-Abd antibody (Clone 28-16-8S) (ABIN114309) specifically detects MHC Class II I-Abd in CyTox.
The antibody is reactive with 小鼠 samples.
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产品编号 ABIN114309
发货至:
中国
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Our Local Distributor
中国
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Quick Overview for MHC Class II I-Abd 抗体 (ABIN114309)
抗原
MHC Class II I-Abd
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Cytotoxicity Test (CyTox)
克隆位点
28-16-8S
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纯化方法
- Affinity chromatography
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免疫原
- C3H.SW spleen Donor: C3H Fusion Partner: SP2/0Ag.14
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亚型
- IgM
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anti-MHC Class II I-Abd antibody (Biotin)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 28-16-8S Biotin
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应用备注
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Flow Cytometry. Can be used to quantitate or eliminate cells bearing the I-Ab antigen from the appropriatestrains of mice.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1. 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 640 and mix.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS and contains 0.02 % sodium azide (NaN3) as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II I-Abd
抗原
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