MHC Class II I-Abd 抗体 (Biotin)
This 小鼠 单克隆 antibody specifically detects MHC Class II I-Abd in FACS. It exhibits reactivity toward 小鼠.
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产品编号 ABIN114307
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MHC Class II I-Abd 抗体 (Biotin) (ABIN114307)
抗原
MHC Class II I-Abd
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
28-16-8S
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纯化方法
- Affinity chromatography
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亚型
- IgM
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anti-MHC Class II I-Abd antibody
适用: 小鼠 CyTox 宿主: 小鼠 Monoclonal 28-16-8S unconjugated
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应用备注
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Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2µg - 0. 5mg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS and contains 0.02 % sodium azide (NaN3) as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II I-Abd
抗原
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