Galectin 3 抗体 (Biotin)
Quick Overview for Galectin 3 抗体 (Biotin) (ABIN114276)
抗原
See all Galectin 3 (LGALS3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- Plasma membrane glycoproteins from C57BL/6 mouse thioglycollateelicited peritoneal exudate
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亚型
- IgG2a
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anti-Galectin 3 (LGALS3) (AA 1-250) antibody
LGALS3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Galectin 3 (LGALS3) (AA 1-250) antibodyLGALS3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Galectin 3 (LGALS3) antibodyLGALS3 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Galectin 3 (LGALS3) antibodyLGALS3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Galectin 3 (LGALS3) (AA 1-250) antibodyLGALS3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-Galectin 3 (LGALS3) (AA 151-250) antibodyLGALS3 适用: 人, 小鼠 ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Galectin 3 (LGALS3) (AA 1-262) antibodyLGALS3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Galectin 3 (LGALS3) antibodyLGALS3 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Monoclonal 4E1 unconjugated Recombinant Antibody
anti-Galectin 3 (LGALS3) (AA 1-250) antibodyLGALS3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Galectin 3 (LGALS3) (AA 2-250) antibodyLGALS3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ABIN114276 can be used for Indirect Immunofluorescence staining, including flow cytometricanalysis of live cells. The addition of propidium iodide is optional, its use eliminatesstaining artifacts caused by dead cells (1). This clone is also suitable for frozen sections(4,5) and ELISA (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 2 µg* of ABIN114276 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a dilution recommended by themanufacturer. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.02 % Sodium Azide as preservative and EIA Grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in alqiuots) at -20 °C.
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- Galectin 3 (LGALS3)
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别名
- Galectin-3
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物质类
- Chemical
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背景
- Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.Synonyms: 35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Lgals3, Mac-2
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基因ID
- 10090
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UniProt
- P16110
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途径
- RTK signaling
抗原
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