CD72 抗体 (FITC)
Quick Overview for CD72 抗体 (FITC) (ABIN114238)
抗原
See all CD72 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with the CD72 alloantigen CD72.1, a B-cell surface protein that is encoded by the Cd72a allele. CD72.1 is expressed on cells of the B cell lineage, except plasma cells3. Mouse strains expressing CD72.1 include C57L/-, C58/-, DBA/1, DBA/2, and SWR/J. Tissue Distribution by Flow Cytometry Analysis: (Representative Dot Plot) Mouse Strain Tested: DBA/2 Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 0.25 μg/10e6 cells Isotypic Control: FITC Mouse IgG2a.
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亚型
- IgG2a
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anti-CD72 Molecule (CD72) antibody
CD72 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal 3F3 unconjugated
anti-CD72 Molecule (CD72) (AA 117-359) antibodyCD72 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 4G4B1 unconjugated
anti-CD72 Molecule (CD72) (AA 117-359) antibodyCD72 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2F3B8 unconjugated
anti-CD72 Molecule (CD72) (AA 169-359) antibodyCD72 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) (AA 143-345) antibodyCD72 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) (AA 126-237) antibodyCD72 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) antibodyCD72 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal BU40 unconjugated
anti-CD72 Molecule (CD72) (AA 4-110) antibodyCD72 适用: 人 FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) (AA 150-208) antibodyCD72 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) antibodyCD72 适用: 人 WB, FACS, IP, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal Bu40 unconjugated
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应用备注
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Flow cytometry (For details please see protocols / specifity below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~0. 25 µg of antibody per 1 x 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.09 % sodium azide (NaN3) and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD72 (CD72 Molecule (CD72))
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别名
- CD72
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背景
- CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32
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基因ID
- 100504743
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NCBI登录号
- XP_003086403
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UniProt
- P21855
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途径
- BCR Signaling
抗原
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