Specific for the amino terminal of eNOS. It recognizes the non-myrisylated form best, but does cross-react ~50 % with the myristylated form of eNOS. Also recognizes Rat eNOS. Cross-reactivity: eNOS (2-11 Cys12): 100 % Recombinant eNOS (NOS type III): 100 % eNOS (cap-2-11Cys12): 50 % Recombinant myristylated eNOS (myristylated NOS type III): 0 % Recombinant hnNOS (NOS type I): 0 % Recombinant hiNOS (NOS type II): 0 %
交叉反应 (详细)
Species reactivity (tested):Human and Rat.
免疫原
eNOS (2-11 Cys12) covalently attached to a carrier protein
Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS), inducible NOS (iNOS) or macrophage NOS (mNOS), and endothelial NOS (eNOS). eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial