Gives a single arc when tested by IEP against fresh Human plasma. Identity has been confirmed by Double Diffusion against fresh Human plasma and a known anti-Human C5.
C5
适用: 人
WB, IA
宿主: 小鼠
Monoclonal
568
unconjugated
应用备注
Immunohistochemistry: 1/20-1/200 (as a guide). Immunodiffusion. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
缓冲液
Glycine buffered saline, pH 7.4, containing 0.09 % Sodium Azide, 0.1 % EACA, 0.01 % Benzamidine and 1 mM EDTA
储存液
Sodium azide, Benzamidine, EACA
注意事项
This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid multiple freeze/thaw cycles.
C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5