Cardiotin 抗体
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- 抗原
- Cardiotin
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适用
- 人, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody SR-4 reacts with Cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues. SR-4 recognizes exclusively the 300 kDa Cardiotin protein complex by Immunoblotting.
- 交叉反应 （详细）
- Species reactivity (tested):Human and Swine.
- 纯化方法
- Purified
- 免疫原
- Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cardiotin.
- 克隆位点
- SR-4
- 亚型
- IgM
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anti-Cardiotin antibody
适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 大鼠, 非洲爪蟾, 小鼠, 斑马鱼, 兔 WB, IHC (fro), IHC, IHC (p) 宿主: 小鼠 Monoclonal R2G unconjugated
anti-Cardiotin antibody适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 大鼠, 非洲爪蟾, 兔 WB, IHC (fro), IHC 宿主: 小鼠 Monoclonal R2G unconjugated
anti-Cardiotin antibody适用: 人, Pig WB, IHC (fro), IHC, FACS 宿主: 小鼠 Monoclonal SR-4 unconjugated
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- 应用备注
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Suitable for Immunohistochemistry on frozen sections (1/25-1/50 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent), Immunoblotting (1/25-1/250)and Flow cytometry (1/25-1/50).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Cardiotin
- 背景
- Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
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