Cardiotin 抗体
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- 抗原
- Cardiotin
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适用
- 人, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody SR-2 reacts with Cardiotin, a mitochondrionassociated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro. In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Swine.
- 纯化方法
- Purified
- 免疫原
- 100 kDa cardiotin subunit
- 克隆位点
- SR-2
- 亚型
- IgM
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anti-Cardiotin antibody
适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 大鼠, 非洲爪蟾, 小鼠, 斑马鱼, 兔 WB, IHC (fro), IHC, IHC (p) 宿主: 小鼠 Monoclonal R2G unconjugated
anti-Cardiotin antibody适用: 人, Pig, Cat, 犬, 山羊, Hamster, 猴, 大鼠, 非洲爪蟾, 兔 WB, IHC (fro), IHC 宿主: 小鼠 Monoclonal R2G unconjugated
anti-Cardiotin antibody适用: 人, Pig WB, IHC (fro), IHC, FACS 宿主: 小鼠 Monoclonal SR-4 unconjugated
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- 应用备注
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Immunohistochemistry on frozen and paraffin-embedded tissue (1: 25 - 1: 100, withavidinbiotinylated horseradish peroxidase complex (ABC) as detection reagent). Immunoblotting (1: 50 - 1: 500, reacts with the 300 kDa cardiotin protein complex and its100 kDa and 60 kDa subunits).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Cardiotin
- 背景
- Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
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