CTNNA3 抗体 (N-Term)
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- 抗原 See all CTNNA3 抗体
- CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))
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抗原表位
- AA 164-177, N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CTNNA3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
- 特异性
- The 892_24D2S antibody against αT-Catenin does not show any cross reaction with αE-Catenin or αN-Catenin in western blot analysis. This 892_24D2S antibody reacts with an epitope located between residues 164-177 of the Human αT-Catenin protein.
- 纯化方法
- Purified
- 免疫原
- Synthetic peptide corresponding to amino acids 164-177 of the human alphaT-catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin.
- 克隆位点
- 892_24D2S
- 亚型
- IgG2a
- Top Product
- Discover our top product CTNNA3 Primary Antibody
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anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) (AA 1-240) antibody
CTNNA3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) (AA 1-516) antibodyCTNNA3 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) (AA 1-516) antibodyCTNNA3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) antibodyCTNNA3 WB, IP, ICC, FACS 宿主: 小鼠 Monoclonal 892_24D2S unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) (AA 1-516) antibodyCTNNA3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) (AA 1-376) antibodyCTNNA3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F4-1B11 unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) (AA 163-302) antibodyCTNNA3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) antibodyCTNNA3 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3) (AA 164-177) antibodyCTNNA3 适用: 人 WB, IHC, IF, IP, ICC 宿主: 小鼠 Monoclonal 892_24D2S unconjugated
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- 应用备注
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Immunofluorescence: aceton-fixed cells. Immunocytochemistry: methanol and paraformaldehyde-fixed cells, followed bypermeabilisation in 0.2 % Triton-X 100 for 2 min. Immunohistochemistry1: 1/25-1/200 with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent,Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS with 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store lyophilized at 2-8 °C and reconstituted (in aliquots) at -20 °C.
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- 抗原
- CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))
- 别名
- Catenin alpha-3 / CTNNA3 (CTNNA3 产品)
- 背景
- Catenins are proteins that connect cadherin/β- catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-catenins to which it shows an overall amino acid identity of 57 % . In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by coimmunoprecipitation.Synonyms: Alpha Catenin 3, Alpha T-catenin, Cadherin-associated protein
- 基因ID
- 29119
- NCBI登录号
- NP_001120856
- UniProt
- Q9UI47
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