Keratin 6 抗体
(1 reference)Quick Overview for Keratin 6 抗体 (ABIN112170)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- The antibody is suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e. g. poorly differentiated adenocarcinoma. Clone Ks6.KA12 represents an excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions). Reacts with a Mr 56000 polypeptide (keratin K6, formerly also designated cytokeratin 6) of squamous epithelia. Tested Reactivities on Cultured Cell Lines: A-431, Detroit 562 and SCC-12.
-
纯化方法
- Affinity Chromatography on Protein A.
-
免疫原
- Keratin K6 of Human callus cytoskeletal preparation.
-
亚型
- IgG1
-
-
anti-Keratin 6 (KRT6) antibody
KRT6 适用: 人, 小鼠, 大鼠 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Keratin 6 (KRT6) antibodyKRT6 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-606R unconjugated Recombinant Antibody
anti-Keratin 6 (KRT6) antibodyKRT6 适用: 人 WB 宿主: 小鼠 Monoclonal 4A1 unconjugated
anti-Keratin 6 (KRT6) (C-Term) antibodyKRT6 适用: 人, 小鼠 IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal LHK6 unconjugated
-
-
应用备注
-
Immunoblotting (Western blot). Immunohistochemistry on Frozen and Paraffin Embedded Sections after formalin fixation(microwave treatment improves performance). After reconstitution, dilute the antibodyfurther 1/10 with PBS for Immunohistochemical applications. Incubation Time: 1h at RT, extended with Paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore in 1 mL distilled water.
-
缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freeze-thaw cycles.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
-
: "Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 5, pp. 1105-16, (2009) (PubMed).
-
: "Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 5, pp. 1105-16, (2009) (PubMed).
-
- Keratin 6 (KRT6)
-
别名
- Cytokeratin 6
-
背景
- Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation related keratins). Keratin 6 is found in hair follicles, neck squamous cell carcinomas, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. There are at least six isoforms of human type II keratin 6 (K6), K6A being the most abundant representing about 77 % of all forms found in epithelia.Synonyms: Cytokeratin 6, Cytokeratin6A, Cytokeratin6D, K6, KRT-6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E, KRTL1, Keratin type II cytoskeletal 6A
-
基因ID
- 3853
-
NCBI登录号
- NP_005545
-
UniProt
- P02538
抗原
-
