Desmoglein 2 抗体
(1 reference)-
- 抗原 See all Desmoglein 2 (DSG2) 抗体
- Desmoglein 2 (DSG2)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Desmoglein 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- The antibody recognizes Desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide Reacting: Desmoglein 2 (1069aa, calculated Mol. Wt. 116,760). Reactivity on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7. Negative with Mouse and Rat.
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)
- 克隆位点
- 10G11
- 亚型
- IgG1
- Top Product
- Discover our top product DSG2 Primary Antibody
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anti-Desmoglein 2 (DSG2) antibody
DSG2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) (AA 72-121) antibodyDSG2 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) (N-Term) antibodyDSG2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) (N-Term) antibodyDSG2 适用: 人, 大鼠, 小鼠, Cow, 犬, Pig, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) (AA 275-533) antibodyDSG2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) (AA 183-331) antibodyDSG2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) (AA 37-164) antibodyDSG2 适用: 人 WB, IF, IP, ICC 宿主: 小鼠 Monoclonal 10D2 unconjugated
anti-Desmoglein 2 (DSG2) (AA 51-150) antibodyDSG2 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) antibodyDSG2 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 2 (DSG2) antibodyDSG2 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 6D8 unconjugated
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- 应用备注
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ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Frozen Sections: For better resolution preincubation (directlyafter fixation) with 0.05-0.2 % Triton X-100 for 5-10 min, depending on tissue type, isrecommended (see Ref.3). Immunohistochemistry on Paraffin Sections: Use microwave treatment. Incubation Time: 1 h at RT for Frozen Tissue and overnight at 2-8 °C for Paraffin Sections. Working Dilution: Dilute 1/10 with PBS, pH 7.4 for Immunohistochemical applications . Recommended Positive Control: CaCo-2, HaCaT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in 1 mL distilled water
- 缓冲液
- Final solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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: "Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 298, Issue 5, pp. G774-83, (2010) (PubMed).
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: "Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 298, Issue 5, pp. G774-83, (2010) (PubMed).
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- 抗原
- Desmoglein 2 (DSG2)
- 别名
- Desmoglein-2 (DSG2 产品)
- 别名
- DSG2 antibody, dsc antibody, dsga antibody, si:dkeyp-51f12.6 antibody, wu:fj23f02 antibody, ARVC10 antibody, ARVD10 antibody, CDHF5 antibody, CMD1BB antibody, HDGC antibody, AA408168 antibody, D18Ertd293e antibody, desmoglein 2 antibody, desmoglein 2, tandem duplicate 1 antibody, DSG2 antibody, dsg2.1 antibody, dsg2 antibody, Dsg2 antibody
- 背景
- Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.Synonyms: CDHF5, Cadherin family member 5, DSG2, HDGC
- 基因ID
- 1829
- NCBI登录号
- NP_001934
- UniProt
- Q14126
- 途径
- Cell-Cell Junction Organization
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