MHC Class II Antigen I Ak,d,b,q,r 抗体 (FITC)
This anti- antibody is a 大鼠 单克隆 antibody detecting in FACS 和 IHC (fro). Suitable for 小鼠.
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Quick Overview for MHC Class II Antigen I Ak,d,b,q,r 抗体 (FITC) (ABIN111989)
抗原
MHC Class II Antigen I Ak,d,b,q,r
适用
小鼠
宿主
大鼠
克隆类型
单克隆
标记
FITC
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
ER-TR3
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无交叉反应
- 人
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交叉反应 （详细）
- Species reactivity (tested):Mouse (Cells expressing MHC class II antigens.
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纯化方法
- Affinity Chromatography.
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免疫原
- Murine thymic reticulum. Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.
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亚型
- IgG2b
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anti-MHC Class II Antigen I Ak,d,b,q,r antibody (Biotin)
适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal ER-TR3 Biotin
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应用备注
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Flow Cytometry: 10 μg/mL (Use 10 μL of 1/10 diluted antibody to label 10^6 cells). Suggested Positive Control: mouse spleen. Has beed described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- Phosphate buffered saline pH 7.2 (PBS) containing 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- MHC Class II Antigen I Ak,d,b,q,r
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别名
- MHC Class II (I-A K,b,d,q,r)
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背景
- MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
抗原
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