MHC Class II Antigen I Ak,d,b,q,r 抗体
适用: 小鼠
FACS, IHC (fro)
宿主: 大鼠
Monoclonal
ER-TR3
unconjugated
-
- 抗原
- MHC Class II Antigen I Ak,d,b,q,r
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 无交叉反应
- 人
- 交叉反应 （详细）
- Species reactivity (tested):Mouse (Cells expressing MHC class II antigens).
- 纯化方法
- Affinity Chromatography
- 免疫原
- Murine thymic reticulum. Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.
- 克隆位点
- ER-TR3
- 亚型
- IgG2b
-
anti-MHC Class II Antigen I Ak,d,b,q,r antibody (Biotin)
适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal ER-TR3 Biotin
anti-MHC Class II Antigen I Ak,d,b,q,r antibody (FITC)适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal ER-TR3 FITC
-
- 应用备注
-
Immunohistochemistry on Frozen Sections: 1.5 μg/mL (1/200). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been reported to work in Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with Frozen, ice-cold Acetone-Fixed Sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- 限制
- 仅限研究用
-
- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.3 mg/mL
- 缓冲液
- Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- 抗原
- MHC Class II Antigen I Ak,d,b,q,r
- 别名
- MHC Class II (I-A K,b,d,q,r)
- 背景
- MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
-
You are here:
