MHC Class II Antigen I Ak,d,b,q,r 抗体 (FITC)
适用: 小鼠
FACS, IHC (fro)
宿主: 大鼠
Monoclonal
ER-TR3
FITC
1 image
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- 抗原
- MHC Class II Antigen I Ak,d,b,q,r
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 无交叉反应
- 人
- 交叉反应 （详细）
- Species reactivity (tested):Mouse (Cells expressing MHC class II antigens.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Murine thymic reticulum. Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.
- 克隆位点
- ER-TR3
- 亚型
- IgG2b
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anti-MHC Class II Antigen I Ak,d,b,q,r antibody (Biotin)
适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal ER-TR3 Biotin
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- 应用备注
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Flow Cytometry: 10 μg/mL (Use 10 μL of 1/10 diluted antibody to label 10^6 cells). Suggested Positive Control: mouse spleen. Has beed described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- Phosphate buffered saline pH 7.2 (PBS) containing 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C.
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- 抗原
- MHC Class II Antigen I Ak,d,b,q,r
- 别名
- MHC Class II (I-A K,b,d,q,r)
- 背景
- MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
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