CD46 抗体
(2 references)-
- 抗原 See all CD46 抗体
- CD46
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD46 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity Chromatography
- 免疫原
- Human U-251 cells.
- 克隆位点
- 13-42
- 亚型
- IgG1
- Top Product
- Discover our top product CD46 Primary Antibody
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anti-CD46 (CD46) (AA 40-320) antibody
CD46 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD46 (CD46) (AA 35-179) antibodyCD46 适用: 人 WB, FACS, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 1A5A5 unconjugated
anti-CD46 (CD46) (AA 317-343) antibodyCD46 适用: 人 WB, FACS, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD46 (CD46) (AA 251-355) antibodyCD46 适用: 人, 小鼠 WB, FACS, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD46 (CD46) antibodyCD46 适用: 人, Cow WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-258 unconjugated
anti-CD46 (CD46) antibodyCD46 适用: 人, Cow WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-258 unconjugated
anti-CD46 (CD46) antibodyCD46 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 7D12 unconjugated Recombinant Antibody
anti-CD46 (CD46) antibody (FITC)CD46 适用: 人, Cow FACS 宿主: 小鼠 Monoclonal MEM-258 FITC
anti-CD46 (CD46) antibody (Biotin)CD46 适用: 人, Cow WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-258 Biotin
anti-CD46 (CD46) antibody (APC)CD46 适用: 人, Cow FACS 宿主: 小鼠 Monoclonal MEM-258 APC
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- 应用备注
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Suitable for Immunohistochemistry (IHC), FACS, Western Blots and Functional Studies. Recommended Dilutions: IHC on Frozen sections: 1-2 μg/mL (1/200-1/400)IHC on Paraffin sections: 8 μg/mL (1/50), Proteinase K pretreatment for antigen retrieval isrecommended. Flow Cytometry: 5-10 μg/mL of neat antibody to label 1x10^6 cells. Immunoprecipitation: 5 μg/ 200 μL extract from 2x10^6 cells. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- Stock solution contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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: "Immunohistochemical localization of complement regulatory proteins in the human retina." in: Histology and histopathology, Vol. 27, Issue 3, pp. 357-64, (2012) (PubMed).
: "Induction of cell-cell fusion from without by human herpesvirus 6B." in: Journal of virology, Vol. 80, Issue 19, pp. 9916-20, (2006) (PubMed).
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: "Immunohistochemical localization of complement regulatory proteins in the human retina." in: Histology and histopathology, Vol. 27, Issue 3, pp. 357-64, (2012) (PubMed).
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- 抗原
- CD46
- 别名
- CD46 / MCP (CD46 产品)
- 别名
- AHUS2 antibody, MCP antibody, MIC10 antibody, TLX antibody, TRA2.10 antibody, Mcp antibody, CD46 antibody, CD46 molecule antibody, CD46 antigen, complement regulatory protein antibody, CD46 molecule, complement regulatory protein antibody, membrane cofactor protein-like antibody, CD46 antibody, Cd46 antibody, LOC100341359 antibody, LOC103789310 antibody
- 背景
- CD46 is also known as Membrane Cofactor Protein (MCP). CD46 acts as a cofactor for factor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complement activation proteins. CD46 is composed of an amino terminus of four short consensus repeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknown function, a transmembrane region, and a cytoplasmic tail. There are many isoforms on human cells and in body fluids which are distinguishable by SDS-PAGE. Their heterogeneity stems from varying amounts of O-linked sugars secondary to alternative splicing of mRNA coding the ST-rich region.Synonyms: MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen
- 基因ID
- 9606
- UniProt
- P15529
- 途径
- Regulation of Actin Filament Polymerization
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