CD46 抗体
(2 references)-
- 抗原 See all CD46 抗体
- CD46
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD46 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity Chromatography
- 免疫原
- Human U-251 cells.
- 克隆位点
- 13-42
- 亚型
- IgG1
- Top Product
- Discover our top product CD46 Primary Antibody
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anti-CD46 (CD46) (AA 40-320) antibody
CD46 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD46 (CD46) (AA 35-179) antibodyCD46 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 1A5A5 unconjugated
anti-CD46 (CD46) (AA 35-179) antibodyCD46 适用: 人 WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 5A8C11 unconjugated
anti-CD46 (CD46) (AA 317-343) antibodyCD46 适用: 人 WB, FACS, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD46 (CD46) (AA 251-355) antibodyCD46 适用: 人, 小鼠 WB, FACS, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD46 (CD46) antibodyCD46 适用: 人, Cow WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-258 unconjugated
anti-CD46 (CD46) antibodyCD46 适用: 人, Cow WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-258 unconjugated
anti-CD46 (CD46) antibodyCD46 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 7D12 unconjugated Recombinant Antibody
anti-CD46 (CD46) antibody (Biotin)CD46 适用: 人, Cow WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-258 Biotin
anti-CD46 (CD46) antibody (FITC)CD46 适用: 人, Cow FACS 宿主: 小鼠 Monoclonal MEM-258 FITC
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- 应用备注
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Suitable for Immunohistochemistry (IHC), FACS, Western Blots and Functional Studies. Recommended Dilutions: IHC on Frozen sections: 1-2 μg/mL (1/200-1/400)IHC on Paraffin sections: 8 μg/mL (1/50), Proteinase K pretreatment for antigen retrieval isrecommended. Flow Cytometry: 5-10 μg/mL of neat antibody to label 1x10^6 cells. Immunoprecipitation: 5 μg/ 200 μL extract from 2x10^6 cells. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- Stock solution contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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: "Immunohistochemical localization of complement regulatory proteins in the human retina." in: Histology and histopathology, Vol. 27, Issue 3, pp. 357-64, (2012) (PubMed).
: "Induction of cell-cell fusion from without by human herpesvirus 6B." in: Journal of virology, Vol. 80, Issue 19, pp. 9916-20, (2006) (PubMed).
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: "Immunohistochemical localization of complement regulatory proteins in the human retina." in: Histology and histopathology, Vol. 27, Issue 3, pp. 357-64, (2012) (PubMed).
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- 抗原
- CD46
- 别名
- CD46 / MCP (CD46 产品)
- 背景
- CD46 is also known as Membrane Cofactor Protein (MCP). CD46 acts as a cofactor for factor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complement activation proteins. CD46 is composed of an amino terminus of four short consensus repeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknown function, a transmembrane region, and a cytoplasmic tail. There are many isoforms on human cells and in body fluids which are distinguishable by SDS-PAGE. Their heterogeneity stems from varying amounts of O-linked sugars secondary to alternative splicing of mRNA coding the ST-rich region.Synonyms: MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen
- 基因ID
- 9606
- UniProt
- P15529
- 途径
- Regulation of Actin Filament Polymerization
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