CD33 抗体
Quick Overview for CD33 抗体 (ABIN111914)
抗原
See all CD33 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The CD33 antibody is valuable for distinguishing acute myeloid from lymphoblastic leukaemias. CD33 is expressed on monocytes in peripheral blood. In the bone marrow, CD33 antigen is found on granulocyte and macrophage precursors, and is absent from pluripotent stem cells.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography
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亚型
- IgG2b
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anti-CD33 (CD33) (AA 101-200) antibody
CD33 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), ICC, IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 15-237) antibodyCD33 适用: 人 FACS, WB, ELISA, IHC 宿主: 小鼠 Monoclonal 2C6B7 unconjugated
anti-CD33 (CD33) (AA 15-237) antibodyCD33 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 2C6B7 unconjugated
anti-CD33 (CD33) (AA 18-240) antibodyCD33 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 49-259) antibodyCD33 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 261-364) antibodyCD33 适用: 人 FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) (AA 1-364) antibodyCD33 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2D12-G4 unconjugated
anti-CD33 (CD33) antibodyCD33 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD33 (CD33) antibodyCD33 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, MS 宿主: 小鼠 Monoclonal WM53 unconjugated
anti-CD33 (CD33) antibodyCD33 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal WM53 unconjugated
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应用备注
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Immunohistochemistry (IHC) on frozen sections: 4 μg/mL (1: 100). FACS: 10 l stock solution to label 10^6 cells. Immunoprecipitation. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- PBS, pH 7.2, 0.05 % (v/v) Kathon CG, 5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C
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储存方法
- Store the antibody at 2-8 °C after reconstitution.
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- CD33
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别名
- CD33 / SIGLEC3
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背景
- CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67 kDa single chain transmembrane glycoprotein that belongs to the sialoadhesin family.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
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基因ID
- 945
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NCBI登录号
- NP_001763
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UniProt
- P20138
抗原
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