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- 抗原 See all CD163 抗体
- CD163
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适用
- 人, 猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD163 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody is specific for CD163. Clone RM 3/1 recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Does not react with lymphocytes, granulocytes or platelets. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with clone 25F9 which is not the case in other tissues.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Monkey. Negative in Pig, Rabbit and Guinea Pig.
- 纯化方法
- Affinity Chromatography
- 免疫原
- Human monocytes, particularly after dexamethasone treatment or after 2-5 days in culture
- 克隆位点
- RM3-1
- 亚型
- IgG1
- Top Product
- Discover our top product CD163 Primary Antibody
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anti-CD163 (CD163) (AA 1001-1121) antibody
CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1001-1121) antibody (FITC)CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 IHC, ELISA, ISt, Coat, StM 宿主: 小鼠 Monoclonal M130-2162 unconjugated
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal M130-1210 unconjugated
anti-CD163 (CD163) (AA 42-259) antibodyCD163 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7G5E2 unconjugated
anti-CD163 (CD163) (AA 697-972) antibodyCD163 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) antibodyCD163 适用: 人 WB, FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal GHI-61 unconjugated
anti-CD163 (CD163) (AA 42-259) antibodyCD163 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 8B7D11 unconjugated
anti-CD163 (CD163) (AA 47-201) antibodyCD163 适用: 人, 小鼠, 大鼠 WB, FACS, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1092-1109), (C-Term) antibodyCD163 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Does not work in routinely processed paraffin sections. Has been described to work in FACS and Western blots. Recommended Positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water to a concentration of 0.4 mg/mL.
- 浓度
- 0.4 mg/mL
- 缓冲液
- Stock solutions contains PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted Prior to and after reconstitution at 2-8 °C. DO NOT FREEZE!
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- 抗原
- CD163
- 别名
- CD163 (CD163 产品)
- 别名
- DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody
- 背景
- CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
- 基因ID
- 9332
- NCBI登录号
- NP_004235
- UniProt
- Q86VB7
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