CD163 抗体
Quick Overview for CD163 抗体 (ABIN111834)
抗原
See all CD163 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises the Rat CD163 cell surface glycoprotein, a 175 kDa molecule also known as ED2. It does not react with Human.
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交叉反应 （详细）
- Species reactivity (tested):Rat.
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.
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亚型
- IgG1
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anti-CD163 (CD163) (AA 1001-1121) antibody
CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1001-1121) antibody (FITC)CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 IHC, ELISA, ISt, Coat, StM 宿主: 小鼠 Monoclonal M130-2162 unconjugated
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal M130-1210 unconjugated
anti-CD163 (CD163) (AA 42-259) antibodyCD163 适用: 人, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 7G5E2 unconjugated
anti-CD163 (CD163) (AA 697-972) antibodyCD163 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) antibodyCD163 适用: 人 WB, FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal GHI-61 unconjugated
anti-CD163 (CD163) (AA 47-201) antibodyCD163 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1092-1109), (C-Term) antibodyCD163 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 693-939) antibodyCD163 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted CD163 antibody to label 10^6 cells in 100μL. Immunohistochemistry on Frozen Sections: 1/50-1/100Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval usingpronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation(Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5. )Histology Positive Control Tissue: liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS with 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD163
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别名
- CD163
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背景
- CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
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基因ID
- 9332
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NCBI登录号
- NP_004235
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UniProt
- Q86VB7
抗原
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