Clathrin Heavy Chain (CLTC) (Heavy Chain) 抗体
Quick Overview for Clathrin Heavy Chain (CLTC) (Heavy Chain) 抗体 (ABIN111496)
抗原
See all Clathrin Heavy Chain (CLTC) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- Heavy Chain
-
特异性
- Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.
-
交叉反应 （详细）
- Species reactivity (tested):Human, Bovine, Pig, Rat and Amphibia.
-
纯化方法
- Gel filtration
-
免疫原
- Coated vesicles (Clathrin) of Bovine brain.
-
亚型
- IgM
-
-
anti-Clathrin Heavy Chain (CLTC) antibody
CLTC 适用: 人 WB, IF, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) antibodyCLTC 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) antibodyCLTC 适用: 人, 小鼠, 大鼠, Cow, Pig WB, ELISA, FACS, IP, ICC 宿主: 小鼠 Monoclonal BF-06 unconjugated
anti-Clathrin Heavy Chain (CLTC) (N-Term) antibodyCLTC 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, Avian, 猴, 兔, 小鸡, 马, Cat, 驴, 山羊, 豚鼠, Hamster, 绵羊 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 1617-1630) antibodyCLTC 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 非洲爪蟾, 猴, 兔, 小鸡, 马, 斑马鱼, Bat IHC, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 232-340) antibodyCLTC 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2E5 unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 401-500) antibodyCLTC 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 851-990) antibodyCLTC 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) (AA 232-340) antibodyCLTC 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Clathrin Heavy Chain (CLTC) antibodyCLTC 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 1 mL distilled water.
-
储存条件
- 4 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C.
-
-
- Clathrin Heavy Chain (CLTC)
-
别名
- Clathrin Heavy Chain
-
背景
- Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker
-
基因ID
- 281080
-
NCBI登录号
- NP_776448
-
UniProt
- P49951
抗原
-
