MAP2 抗体 (Biotin)
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Quick Overview for MAP2 抗体 (Biotin) (ABIN111292)
抗原
See all MAP2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons.
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纯化方法
- Protein G chromatography
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免疫原
- Bovine brain microtubule associated protein 2.
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亚型
- IgG1
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应用备注
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Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. WesternBlotting: 1-2 μg/mL for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mMcitrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant braintissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0 % BSA and 0.09 % sodium azide as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
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- MAP2 (Microtubule-Associated Protein 2 (MAP2))
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别名
- MAP-2
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背景
- Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker
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基因ID
- 4133
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NCBI登录号
- NP_002365
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UniProt
- P11137
抗原
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