WB (1:1000-2000), ICC (1:100-500), Flow Cyt (1:10-100). Other applications have not been tested. The optimal dilutions should be determined by end user.
限制
仅限研究用
状态
Liquid
缓冲液
TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
储存液
Sodium azide, Thimerosal (Merthiolate)
储存条件
4 °C/-20 °C
储存方法
Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.
有效期
12 months
抗原
MCAM
(Melanoma Cell Adhesion Molecule (MCAM))
别名
CD146
背景
CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. 1 CD146 belongs to the immunoglobulin superfamily and expressed in all types of human endothelial cells. The regulated expression of CD146 at areas of cell-cell junction and strongly suggest involvement of CD146 as a mediator of cell-cell interaction. 2 CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, 3 and its expression may be linked to multipotency, mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface. 4