WB (1:1000), ICC (1:50). Other applications have not been tested. The optimal dilutions should be determined by end user.
限制
仅限研究用
状态
Liquid
缓冲液
TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
储存液
Sodium azide, Thimerosal (Merthiolate)
储存条件
4 °C/-20 °C
储存方法
Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.
有效期
12 months
抗原
OCT4 (POU5F1)
(POU Class 5 Homeobox 1 (POU5F1))
别名
Octamer-binding transcription factor 4, OCT-4
背景
Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, class 5, transcription factor 1) is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells. A critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. It has also been showed that Oct3 has a role in cardiac development in the early mouse embryo. Oct-4 expression must be closely regulated, too much or too little will cause differentiation of the cells.