CD63 抗体 (APC)
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- 抗原 See all CD63 抗体
- CD63
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD63 antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD63 APC is recommended for use in flow cytometry for identification of activated platelets.
- 克隆位点
- TEA3-18
- 亚型
- IgG1
- Top Product
- Discover our top product CD63 Primary Antibody
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anti-CD63 (CD63) (AA 120-175) antibody
CD63 适用: 人, 小鼠, 大鼠, 猴, Cow, 犬, 绵羊, 兔, Pig, 马, Hamster, 豚鼠, 山羊, 驴, 小鸡, Cat, Avian FACS, WB, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 unconjugated
anti-CD63 (CD63) antibody (Biotin)CD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 Biotin
anti-CD63 (CD63) antibody (APC)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 APC
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 10F11E6 unconjugated
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 1H1E11 unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人, 小鼠 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal MX-49-129-5 unconjugated
anti-CD63 (CD63) antibody (PE)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PE
anti-CD63 (CD63) antibody (PerCP)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PerCP
anti-CD63 (CD63) antibodyCD63 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 5 µl/ 95 µl PBS.
- 说明
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Allophycocyanin (Febico, Far East Bio-Tech Co.).
- 试剂准备
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A. Procedure for preparation of ACTIVATED PLATELETS 1. Centrifuge tube of freshly drawn EDTA blood (600rpm or 75xg, 20') 2. Remove platelets (top layer) and wash twice over in PBS and resuspend in PBS. 3. Add thrombin to cell suspension (1-0,2 U/ml) or PMA (25µg/ml). 4. Add 20 µl of CD63 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 5. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 6. Incubate in the dark at room temperature at room temperature (20-25 °C) for 15 minutes. 7. Incubate in the dark at 4°C for 10 minutes. 8. Analyse on a flow cytometer. B. Procedure for preparation of RESTING PLATELETS 1. Centrifuge tube of freshly drawn EDTA blood (600rpm or 75xg, 20') 2. Remove platelets (top layer) and wash twice over in PBS and resuspend in PBS. 3. Add 20 µl of CD63 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Analyse on a flow cytometer.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD63
- 别名
- CD63 (CD63 产品)
- 别名
- C75951 antibody, ME491 antibody, Tspan30 antibody, LAMP-3 antibody, MLA1 antibody, OMA81H antibody, TSPAN30 antibody, TSP29FA antibody, CD63 antigen antibody, CD63 molecule antibody, Cd63 molecule antibody, Cd63 antibody, CD63 antibody
- 背景
- CD63 is a 53 kD, type III lysosomal glycoprotein, expressed on activated platelets, monocytes and macrophages. This molecule is also referred highly to as LIMP, gp55,melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a member of the tetraspan transmembrane 4 superfamily (TM4SF). It is also widely expressed on surface and in the cytoplasm of various hematopoietic (monocytes, macrophages) and non-hematopoietic cells (endothelium, fibroblasts, osteoclasts, smooth muscle). Its cellular function has not been fully elucidated. Reports suggest this as a useful molecule to study platelet activation.
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