(1 reference)-
- 抗原 See all CD59 抗体
- CD59
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD59 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Mouse Monoclonal Anti-Human CD59 PE is recommended for use in flow cytometry for enumerates CD59+ lymphocytes in human peripheral blood by flow cytometric methods.
- 克隆位点
- VJ1-12-2
- 亚型
- IgG2a
- Top Product
- Discover our top product CD59 Primary Antibody
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anti-CD59 (CD59) (AA 31-111) antibody
CD59 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8D2B8 unconjugated
anti-CD59 (CD59) (AA 74-110) antibodyCD59 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB56691 unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人 FACS, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-43 unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人, 小鼠 FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-43-5 unconjugated
anti-CD59 (CD59) (AA 31-111) antibodyCD59 适用: 人 FACS, WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-CD59 (CD59) (AA 23-105) antibodyCD59 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) (AA 1-128) antibodyCD59 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G6 unconjugated
anti-CD59 (CD59) (AA 52-100) antibodyCD59 适用: 人 FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) (AA 26-102) antibodyCD59 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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R-Phycoerythrin (Europa Bioproducts, Ely, Cambridge).
- 样品收集
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10 P6 P cells). 2. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 3. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 4. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 5. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 6. Add 20 µl of CD59 PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 7. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 8. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 9. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 10. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 11. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 12. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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: "Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets." in: Cytometry, Vol. 50, Issue 3, pp. 191-201, (2002) (PubMed).
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: "Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets." in: Cytometry, Vol. 50, Issue 3, pp. 191-201, (2002) (PubMed).
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- 抗原
- CD59
- 别名
- CD59 (CD59 产品)
- 别名
- 16.3A5 antibody, 1F5 antibody, EJ16 antibody, EJ30 antibody, EL32 antibody, G344 antibody, HRF-20 antibody, HRF20 antibody, MAC-IP antibody, MACIF antibody, MEM43 antibody, MIC11 antibody, MIN1 antibody, MIN2 antibody, MIN3 antibody, MIRL antibody, MSK21 antibody, p18-20 antibody, Cd59a antibody, Cd59b antibody, MACIP antibody, CD59 molecule (CD59 blood group) antibody, CD59 molecule antibody, CD59 antibody, Cd59 antibody
- 背景
- The antibody reacts with a 19kDa glycosylphosphatidylinositol (GPI) linked glycoprotein expressed on haematopoietic and non-haematopoietic cells. CD59 is expressed, like CD55, in erythrocytes. Recognizes a carbohydrate antigen present on a subset of peripheral blood mononuclear cells involved in the natural killer activity, which is not MHC restricted. The antigen is not present on B cells, monocytes, red blood cells or platelets but is expressed on cells in neuroectodermal tissue. It reacts with 20% of normal human peripheral blood mononuclear cells.
- 途径
- Complement System
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