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MME 抗体 (APC)

Name: MME 抗体 (APC)
Brand: antibodies-online
SKU: ABIN1112037
Availability: OutOfStock

MME 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal HI10a APC

Image no. 1 for anti-Membrane Metallo-Endopeptidase (MME) antibody (APC) (ABIN1112037) (MME 抗体 (APC))

1 image

产品编号 ABIN1112037

发货至: 中国

Datasheet as PDF

抗原 See all MME 抗体

MME (Membrane Metallo-Endopeptidase (MME))
适用
206

58

50

3

2

2

2

2

2

1

1

1

1
人
宿主
111

101

2
小鼠
Compatible Secondaries
克隆类型
108

105

2
单克隆
标记
108

19

18

13

8

6

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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This MME antibody is conjugated to APC
应用范围
132

96

78

67

34

28

22

20

13

13

10

8

6

5

5

3

2

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

产品特性

Monoclonal Mouse Anti-Human CD10 APC is recommended for use in flow cytometry for identification of human common acute lymphoblastic leukaemia antigen (CALLA).

克隆位点

HI10a

亚型

IgG1

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MME 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

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anti-Membrane Metallo-Endopeptidase (MME) antibody (FITC)
MME 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-78 FITC

anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibody
MME 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 6G12E12 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody
MME 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

说明

Allophycocyanin (Febico, Far East Bio-Tech Co.).

样品收集

1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD10 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

限制

仅限研究用
状态

Liquid

缓冲液

The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

储存液

Sodium azide

注意事项

1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

储存条件

4 °C
抗原

MME (Membrane Metallo-Endopeptidase (MME))

别名

CD10 (MME 产品)

别名

CALLA antibody, CD10 antibody, NEP antibody, SFE antibody, 6030454K05Rik antibody, C85356 antibody, Nep antibody, neprilysin antibody, membrane metalloendopeptidase antibody, membrane metallo endopeptidase antibody, membrane metallo-endopeptidase antibody, MME antibody, Mme antibody

物质类

Chemical

背景

CD10 clon HI10a recognizes neutral endopeptidase, a 95 kDa type II transmembrane glycoprotein, which is refered as the Common Acute Lymphoblastic Leukemia Antigen (CALLA) expressed on common acute lymphoblastic leukaemia cells which is seen on early B-lymphocytes and with stem cells of the lymphocyte lineage and immature thymocytes. The antigen is a neutral endopeptidase.

途径

RTK signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration