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Cytokeratin 18 抗体

Name: Cytokeratin 18 抗体
SKU: ABIN111183
Availability: OutOfStock

KRT18 适用: 人, 小鼠, 大鼠, 犬, Pig, Cow, Hamster, 绵羊, 斑马鱼, Trout WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal Ks18-04 unconjugated

(13 references)

产品编号 ABIN111183

发货至: 中国

Datasheet as PDF

抗原 See all Cytokeratin 18 (KRT18) 抗体

Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
适用
240

128

94

41

33

26

21

19

18

18

16

14

14

14

8

7

6

6

3

3

2

1
人, 小鼠, 大鼠, 犬, Pig, Cow, Hamster, 绵羊, 斑马鱼, Trout
宿主
148

124

3

1

1

1
小鼠
Compatible Secondaries
克隆类型
142

135

1
单克隆
标记
146

23

22

10

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1
This Cytokeratin 18 antibody is un-conjugated
应用范围
220

103

86

76

72

55

54

40

38

27

26

19

8

8

7

6

2

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reactivities on cultured cell lines (tested so far): HeLa and MCF-7

交叉反应（详细）

Species reactivity (tested):Human, Mouse, Rat, Bovine, Porcine, Canine, Hamster, Sheep, Fish (Trout) and Zebrafish

纯化方法

Protein A chromatography

免疫原

Human keratin K18 from HeLa cytoskeletal preparation

克隆位点

Ks18-04

亚型

IgG1

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Discover our top product KRT18 Primary Antibody
anti-Keratin 18 (KRT18) (AA 53-150) antibody
KRT18 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 适用: 人 WB, FACS, ELISA, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal DC-10 unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 适用: 哺乳动物 WB, FACS, ELISA, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C-04 unconjugated

anti-Keratin 18 (KRT18) (AA 238-396) antibody
KRT18 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 1-423) antibody
KRT18 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 238-396) antibody
KRT18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated

anti-Keratin 18 (KRT18) (AA 72-380) antibody
KRT18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 102-396) antibody
KRT18 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 1-430) antibody
KRT18 适用: 人 WB, IF, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 2F8 unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT18-834 unconjugated

应用备注

Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
溶解方式

Restore in 1.0 mL distilled water.

缓冲液

PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing). Dilute only prior to immediate use.

储存条件

-20 °C

储存方法

Store the antiserum at -20 °C (aliquot contents to
Zatloukal, Kufferath, Thueringer, Landegren, Zatloukal, Haybaeck: "Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies." in: PLoS ONE, Vol. 9, Issue 5, pp. e96690, (2014) (PubMed).

Trowe, Airik, Weiss, Farin, Foik, Bettenhausen, Schuster-Gossler, Taketo, Kispert: "Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter." in: Development (Cambridge, England), Vol. 139, Issue 17, pp. 3099-108, (2012) (PubMed).

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, (2012) (PubMed).

Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).

Ikenouchi, Matsuda, Furuse, Tsukita: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, (2003) (PubMed).

Schaffeld, Knappe, Hunzinger, Markl: "cDNA sequences of the authentic keratins 8 and 18 in zebrafish." in: Differentiation; research in biological diversity, Vol. 71, Issue 1, pp. 73-82, (2003) (PubMed).

Stumptner, Fuchsbichler, Heid, Zatloukal, Denk: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, (2002) (PubMed).

Bantel, Ruck, Gregor, Schulze-Osthoff: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, (2001) (PubMed).

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, (1995) (PubMed).

Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." in: Immunology, Vol. 77, Issue 4, pp. 543-9, (1993) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, (1983) (PubMed).
抗原

Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

别名

Cytokeratin 18 (KRT18 产品)

背景

Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

基因ID

3875

NCBI登录号

NP_000215

UniProt

P05783

途径

Apoptosis, Caspase Cascade in Apoptosis