CD34 抗体 (APC)
(10 references)Quick Overview for CD34 抗体 (APC) (ABIN1111107)
抗原
See all CD34 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD34 APC
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特异性
- The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.
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无交叉反应
- 绵羊
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交叉反应 （详细）
- Human, Non-Human Primates
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纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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亚型
- IgG1 kappa
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anti-CD34 (CD34) (AA 366-382), (C-Term) antibody
CD34 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 201-300) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 301-385) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔, 小鸡, 山羊 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 36-287) antibodyCD34 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) antibodyCD34 适用: 人, 大鼠 FACS, IHC, StM 宿主: 兔 Monoclonal HPCA1-2598R unconjugated Recombinant Antibody
anti-CD34 (CD34) antibodyCD34 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), Func 宿主: 小鼠 Monoclonal QBEnd-10 unconjugated
anti-CD34 (CD34) antibody (PE)CD34 适用: 人 FACS, ICC 宿主: 小鼠 Monoclonal 4H11[APG] PE
anti-CD34 (CD34) (C-Term) antibodyCD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 151-385) antibodyCD34 适用: 人, 大鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 124-375) antibodyCD34 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
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限制
- 仅限研究用
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溶解方式
- No reconstitution is necessary.
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze.
Avoid prolonged exposure to light. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).
: "Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 13, pp. 5925-30, (2010) (PubMed).
: "Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes." in: Haematologica, Vol. 94, Issue 8, pp. 1160-3, (2009) (PubMed).
: "The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells." in: Journal of translational medicine, Vol. 4, pp. 9, (2006) (PubMed).
: "CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM)." in: The Journal of investigative dermatology, Vol. 124, Issue 6, pp. 1215-24, (2005) (PubMed).
: "Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation." in: Bone marrow transplantation, Vol. 28, Issue 6, pp. 587-95, (2001) (PubMed).
: "The adapter protein CrkL associates with CD34." in: Blood, Vol. 97, Issue 12, pp. 3768-75, (2001) (PubMed).
: "Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system." in: Experimental hematology, Vol. 28, Issue 6, pp. 690-9, (2000) (PubMed).
: "In vitro reconstitution of human B-cell ontogeny: from CD34(+) multipotent progenitors to Ig-secreting cells." in: Blood, Vol. 92, Issue 12, pp. 4509-20, (1999) (PubMed).
: "Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane." in: Blood, Vol. 93, Issue 10, pp. 3379-90, (1999) (PubMed).
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: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).
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- CD34
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别名
- CD34
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背景
- CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
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基因ID
- 947
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UniProt
- P28906
抗原
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