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Vimentin 抗体 (pSer55)

This anti-Vimentin antibody is a 小鼠单克隆 antibody detecting Vimentin in WB 和 IF. Suitable for 人, 小鼠和大鼠.

Western Blotting (WB) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin 抗体 (pSer55))

Immunofluorescence (IF) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin 抗体 (pSer55))

2 images

产品编号 ABIN1109485

发货至: 中国

Quick Overview for Vimentin 抗体 (pSer55) (ABIN1109485)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

适用

376
129
127
63
61
49
43
38
30
22
21
13
13
8
8
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6
3
3
2
1
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人, 小鼠, 大鼠

宿主

199
163
11
6
6
5
2
2

小鼠

克隆类型

单克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

4A4

Discover our top product VIM Primary Antibody

抗原表位
34

32

17

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer55

序列

SLYSS-phosphoS55-PGGAYC-KLH

特异性

This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.

交叉反应（详细）

Species reactivity (tested):Human, mouse, rat

纯化方法

Protein A agarose

免疫原

Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.

亚型

IgG2b
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

储存液

Azide free

注意事项

Avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store (in aliquots) at -20 °C.
抗原

Vimentin (VIM)

别名

Vimentin

背景

Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM

基因ID

7431

NCBI登录号

NP_003371

UniProt

P08670

途径

Caspase Cascade in Apoptosis

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