PLAU 抗体
Quick Overview for PLAU 抗体 (ABIN7101951)
抗原
See all PLAU 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. The antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatogryphy: The affinity purified antibody is obtained by binding to immobilized native Human Urokinase (antigen used for immunization), followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization.
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免疫原
- Extensively purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from Human urine. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies.
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亚型
- IgG
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anti-Plasminogen Activator, Urokinase (PLAU) (AA 60-90), (N-Term) antibody
PLAU 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB04087 unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (C-Term) antibodyVerified PLAU 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (AA 396-426) antibodyPLAU 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (AA 60-90) antibodyPLAU 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (AA 180-433) antibodyPLAU 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (AA 16-115) antibodyPLAU 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) antibodyPLAU 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (AA 396-426), (C-Term) antibodyPLAU 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB04089-04090 unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (AA 17-432) antibodyPLAU 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen Activator, Urokinase (PLAU) (AA 21-173) antibodyPLAU 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL distilled water and add preservative if preferred.
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缓冲液
- PBS with Mannitol
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注意事项
- Avoid repeated freezing and thawing.
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- PLAU (Plasminogen Activator, Urokinase (PLAU))
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别名
- uPA / PLAU
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背景
- This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]Synonyms: U-plasminogen activator, Urokinase, Urokinase-type plasminogen activator, Urokinase-type plasminogen activator chain B, Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A
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基因ID
- 5328
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NCBI登录号
- NP_001138503
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途径
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
抗原
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