GFP 抗体 (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN1107357, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
615
21
10
8
5
3
1
1
1
宿主
小鼠
339
167
81
39
16
9
9
2
克隆类型 (克隆位点)
单克隆 ()
标记
This GFP antibody is un-conjugated
33
29
27
14
13
12
11
10
9
9
8
7
6
6
6
6
6
6
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
521
341
205
157
139
132
94
92
78
24
15
12
11
9
8
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4
3
3
3
3
3
2
2
2
1
1
1
1
选项
Supplier
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Supplier Product No.
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克隆位点 4B10B2
亚型 IgG2a
交叉反应 (详细) Species reactivity (tested):Human.
纯化方法 Purified
Plasmids, Primers & others Plasmids, Primers & others GFP products on genomics-online (e.g. as negative or positive controls)
抗原 Green Fluorescent Protein (GFP)
别名 GFP
物质类 Tag
背景 GFP (Green Fluorescence Protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Synonyms: GFP-Tag, Green fluorescent protein
分子量 27 kDa
应用备注 ELISA: 1/10000.
Western Bloting: 1/500 - 1/2000.
Flow Cytometry: 1/200 - 1/400.
Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
限制 仅限研究用
缓冲液 PBS, 0.03 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107357) anti-Green Fluorescent Protein (GFP) antibody
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107357) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107357) anti-Green Fluorescent Protein (GFP) antibody (Image 3)
Flow Cytometry (FACS) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107357) anti-Green Fluorescent Protein (GFP) antibody (Image 4)
背景 Hill, Nelson, Tilman, Polasky, Tiffany: "Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11206-10, 2006 (PubMed).

Dittman, Kaplan: "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11399-404, 2006 (PubMed).

Arasada, Son, Ramalingam, Eichinger, Schleicher, Rohlfs: "Characterization of the Ste20-like kinase Krs1 of Dictyostelium discoideum." in: European journal of cell biology, Vol. 85, Issue 9-10, pp. 1059-68, 2006 (PubMed).

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