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ARHGEF2 抗体

This 小鼠 单克隆 anti-ARHGEF2 antibody (Clone B4-7) specifically detects ARHGEF2 in WB, IF, IP 和 IHC (fro). The antibody is reactive with 人, 小鼠 和 犬 samples.
产品编号 ABIN1105439
发货至: 中国
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Quick Overview for ARHGEF2 抗体 (ABIN1105439)

抗原

See all ARHGEF2 抗体
ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

适用

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人, 小鼠, 犬

宿主

  • 69
  • 7
  • 2
小鼠

克隆类型

  • 53
  • 25
单克隆

标记

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This ARHGEF2 antibody is un-conjugated

应用范围

  • 60
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  • 7
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  • 1
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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

B4-7
  • 特异性

    The monoclonal antibody B4/7 recognizes guanine nucleotide exchange factor H1 (GEF-H1).

    交叉反应 (详细)

    Species reactivity (tested):Human, canine, mouse

    纯化方法

    Protein G

    亚型

    IgG1
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 2 - 8 °C.
  • 抗原

    ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

    别名

    ARHGEF2

    背景

    GEFH1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of epithelial tight junctions and the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin. GEF-H1 is part of the signaling pathway connecting epithelial polarity with the cell cycle, and as such involved in oncogenesis.Synonyms: GEF-H1, Guanine nucleotide exchange factor H1, KIAA0651, LFP40, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40, Rho guanine nucleotide exchange factor 2

    基因ID

    9181

    NCBI登录号

    NP_001155855

    UniProt

    Q92974

    途径

    Negative Regulation of intrinsic apoptotic Signaling
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