TRAIL 抗体
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北京 101111
Quick Overview for TRAIL 抗体 (ABIN1104738)
抗原
See all TRAIL (TNFSF10) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human TRAIL/Apo2L
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human TRAIL/Apo2L.
Type of Immunogen: Cell extract -
亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.15 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7420 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTRAIL/Apo2L (10 ng/mL), a concentration of 150 ng/mL of this antibody is required. ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTRAIL/Apo2L (LS-C104497) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTRAIL/Apo2L. Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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别名
- TNFSF10 / TRAIL
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背景
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Name/Gene ID: TNFSF10
Family: TNF
Synonyms: TNFSF10, Apo-2L, APO2L, Apo-2 ligand, CD253, CD253 antigen, TRAIL, Protein TRAIL, TL2 -
基因ID
- 8743
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UniProt
- P50591
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途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
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