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PAI1 抗体

This anti-PAI1 antibody is a 兔 多克隆 antibody detecting PAI1 in WB, IHC, ELISA, IHC (p) 和 Neut. Suitable for 人.
产品编号 ABIN1104210
发货至: 中国

Quick Overview for PAI1 抗体 (ABIN1104210)

抗原

See all PAI1 (SERPINE1) 抗体
PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

适用

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克隆类型

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多克隆

标记

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This PAI1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)
  • 品牌

    IHC-plus™

    特异性

    Human PAI-1

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human PAI-1.

    Type of Immunogen: Cell extract

    亚型

    IgG
  • 应用备注

    Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (15 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: LS-B7483 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPAI-1 (4.5 μg/mL), a concentration of at least 15 μg/mL of this antibody is required. ELISA: To detect hPAI-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PAI-1 (LS-C104564) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPAI-1. Western Blot: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

    别名

    SERPINE1 / PAI-1

    背景

    Name/Gene ID: SERPINE1
    Family: Serine Protease Inhibitor

    Synonyms: SERPINE1, PAI1, PLANH1, PAI-1, Serpin E1

    基因ID

    5054

    UniProt

    P05121

    途径

    p53 Pathway, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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