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IL-10 抗体

IL10 适用: 人 WB, ELISA, IHC, Neut, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1102445
发货至: 中国
  • 抗原 See all IL-10 (IL10) 抗体
    IL-10 (IL10) (Interleukin 10 (IL10))
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    多克隆
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    This IL-10 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human IL-10
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human IL-10.

    Type of Immunogen: Cell extract
    亚型
    IgG
    Top Product
    Discover our top product IL10 Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: LS-B7432 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-10 (1 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA: To detect hIL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (LS-C104321) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    IL-10 (IL10) (Interleukin 10 (IL10))
    别名
    IL10 (IL10 产品)
    别名
    CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody
    背景
    Name/Gene ID: IL10
    Family: Hormone

    Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF
    基因ID
    3586
    UniProt
    P22301
    途径
    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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