IL-10 抗体
Quick Overview for IL-10 抗体 (ABIN1102445)
抗原
See all IL-10 (IL10) 抗体适用
宿主
克隆类型
标记
应用范围
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                                            品牌
- IHC-plus™
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                                            特异性
- Human IL-10
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                                            纯化方法
- Immunoaffinity purified
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                                            免疫原
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                        E.coli derived recombinant Human IL-10.
 Type of Immunogen: Cell extract
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                                            亚型
- IgG
 
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                                            应用备注
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                        Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
 Usage: Immunohistochemistry: LS-B7432 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-10 (1 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA: To detect hIL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (LS-C104321) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
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                                            说明
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                        Target Species of Antibody: Human 
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                                            限制
- 仅限研究用
 
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                                            状态
- Lyophilized
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                                            溶解方式
- Sterile water
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                                            浓度
- Lot specific
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                                            缓冲液
- Lyophilized from PBS, pH 7.2
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                                            注意事项
- Avoid freeze/thaw cycles.
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                                            储存条件
- 4 °C,-20 °C
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                                            储存方法
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                        Store Lyophilized at room temperature up to 1 month
 Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
 
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    - IL-10 (IL10) (Interleukin 10 (IL10))
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                                            别名
- IL10
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                                            背景
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                        Name/Gene ID: IL10
 Family: Hormone
 Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF
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                                            基因ID
- 3586
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                                            UniProt
- P22301
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                                            途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
 抗原
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