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IL-10 抗体

This anti-IL-10 antibody is a 兔 多克隆 antibody detecting IL-10 in WB, IHC, ELISA, IHC (p) 和 Neut. Suitable for 人.
产品编号 ABIN1102445
发货至: 中国

Quick Overview for IL-10 抗体 (ABIN1102445)

抗原

See all IL-10 (IL10) 抗体
IL-10 (IL10) (Interleukin 10 (IL10))

适用

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克隆类型

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多克隆

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This IL-10 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)
  • 品牌

    IHC-plus™

    特异性

    Human IL-10

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human IL-10.

    Type of Immunogen: Cell extract

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: LS-B7432 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-10 (1 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA: To detect hIL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (LS-C104321) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    IL-10 (IL10) (Interleukin 10 (IL10))

    别名

    IL10

    背景

    Name/Gene ID: IL10
    Family: Hormone

    Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF

    基因ID

    3586

    UniProt

    P22301

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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