HA-Tag 抗体 (C-Term)
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北京 101111
Quick Overview for HA-Tag 抗体 (C-Term) (ABIN1101941)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- YPYDVPDYA
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特异性
- This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
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纯化方法
- Affinity purified
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过滤
- Sterile filtered
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免疫原
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This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Type of Immunogen: Purified protein -
亚型
- IgG
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anti-HA-Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 18B11H6 unconjugated
anti-HA-Tag antibodyWB, IF, ChIP 宿主: 兔 Polyclonal RB0411 unconjugated
anti-HA-Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibodyWB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag (AA 98-106) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF 宿主: 兔 Monoclonal AB158-1-D11 unconjugated Recombinant Antibody
anti-HA-Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP
anti-HA-Tag antibody适用: 标记 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag (AA 98-106) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF 宿主: 小鼠 Monoclonal AB158-1-D11 unconjugated Recombinant Antibody
anti-HA-Tag antibodyELISA, Coat 宿主: 小鼠 Monoclonal 16-43 unconjugated
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应用备注
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Approved: ChrIP, ELISA, IHC, IHC-P (1:500 - 1:2000), IP, WB (1:2000 - 1:10000)
Usage: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles. Store undiluted.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- HA-Tag
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别名
- Hemagglutinin / HA Tag
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物质类
- Tag
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背景
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Name/Gene ID: HA Tag
Synonyms: HA Tag
抗原
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