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HA-Tag 抗体 (C-Term)

This anti-HA-Tag antibody is a 兔 多克隆 antibody detecting HA-Tag in WB, ELISA, IP, IHC, IHC (p) 和 ChIP. Suitable for .
产品编号 ABIN1101941
发货至: 中国

Quick Overview for HA-Tag 抗体 (C-Term) (ABIN1101941)

抗原

HA-Tag

适用

请咨询

宿主

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克隆类型

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多克隆

标记

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This HA-Tag antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)
  • 抗原表位

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    C-Term

    序列

    YPYDVPDYA

    特异性

    This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

    纯化方法

    Affinity purified

    过滤

    Sterile filtered

    免疫原

    This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.

    Type of Immunogen: Purified protein

    亚型

    IgG
  • 应用备注

    Approved: ChrIP, ELISA, IHC, IHC-P (1:500 - 1:2000), IP, WB (1:2000 - 1:10000)

    Usage: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles. Store undiluted.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
  • 抗原

    HA-Tag

    别名

    Hemagglutinin / HA Tag

    物质类

    Tag

    背景

    Name/Gene ID: HA Tag

    Synonyms: HA Tag
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