Urease 抗体
Quick Overview for Urease 抗体 (ABIN108075)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4182-0100
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Supplier
- Rockland
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原理
- Urease Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
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产品特性
- Synonyms: rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
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纯化方法
- Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Urease [Jack Bean]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Urease (URE) antibody (HRP)
URE 适用: Jack Bean WB, IP, ELISA, DB 宿主: 兔 Polyclonal HRP
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应用备注
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Application Note: Anti-Urease Antibody has been tested in western blot and suitable against 1.0 μg of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Urease (URE)
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别名
- Urease
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背景
- Background: Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism. Urease catalyzes the hydrolysis of urea to produce ammonia and carbamate (ammonia and carbonic acid). Ureases are found in numerous bacteria, fungi, algae, invertebrates, plants, in soils, as a soil enzyme. Due to the catalytic activity producing ammonia the pH of the environment increases. Ureases, when bacterial, can be a mode for the pathogens of many medical conditions such as hepatic encephalopathy, hepatic coma, urinary stones, and peptic ulcers. Urea is found naturally in the environment as well as artificially introduced. Anti-Urease antibody is ideal for researchers interested in ecosystems, microbes, and gut bacteria.
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UniProt
- P07374
抗原
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