RAD9A 抗体 (Ser1260)
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- 抗原 See all RAD9A 抗体
- RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))
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抗原表位
- Ser1260
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RAD9A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 600-401-467
- Supplier
- Rockland
- 原理
- Rad9 S1260 Antibody
- 交叉反应 （详细）
- No reactivity is expected against the human analog of RAD9.
- 产品特性
- Synonyms: Rabbit anti-Rad9 antibody, Rabbit anti-Rad9 S1260 antibody, Rad 9, Rad-9, Cell cycle checkpoint control protein antibody, Cell cycle checkpoint control protein RAD9A antibody, DNA repair exonuclease rad9 homolog A antibody, DNA repair protein RAD9, YDR217C, YD9934.02C
- 纯化方法
- This is an affinity purified antibody directed against yeast Rad9 produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of an internal region near aa 1225-1275 from the aa1309 yeast Rad9 protein conjugated to KLH.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product RAD9A Primary Antibody
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anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 162-391) antibody
RAD9A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (Center) antibodyRAD9A 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) antibodyRAD9A 适用: 人, 猴, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D9 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (pSer328) antibodyRAD9A 适用: 人 WB, IHC (p), DB 宿主: 兔 Polyclonal RB7115 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 267-341) antibodyRAD9A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 29-62), (N-Term) antibodyRAD9A 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52362 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 279-313), (C-Term) antibodyRAD9A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB51320 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) antibodyRAD9A 适用: 人, 小鼠 WB, IP 宿主: 小鼠 Monoclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (C-Term) antibodyRAD9A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (C-Term) antibodyVerified RAD9A 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown. Mature RAD9 is reported to have an apparent molecular weight of ~140 kDa.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))
- 别名
- RAD9 (RAD9A 产品)
- 背景
- Background: Cells respond to DNA damage by activating a network of signaling pathways that control cell cycle progression and DNA repair. Cell cycle checkpoints are mechanisms that transiently delay cell cycle progression when DNA is damaged or DNA replication is incomplete. In the fission yeast Schizosaccharomyces pombe, a group of six such checkpoint control genes have been identified and include rad1+, rad3+, rad9+, rad17+, rad26+ and hus1+. Mutations in any one of these genes render cells sensitive to gamma-rays, UV light or the DNA synthesis inhibitor hydroxyurea (HU) and eliminate the ability of cells to delay entry into mitosis after treatment with these agents. All of these genes apparently link abnormal DNA structures to cell cycle control. As for cell cycle-related genes in general, these checkpoint control genes are highly conserved throughout evolution. Human and mouse versions of several of the S.pombe genes have been isolated, providing strong evidence that checkpoint control mechanisms are also highly conserved. In mammals, these genes are thought to maintain genomic stability, especially in the presence of DNA damage. Therefore, when these genes are altered, genomic instability may occur and lead to cancer. The biochemical activities of most of the checkpoint control gene products are not well established, although progress has been made towards learning more about their function. For example, examination of the structure of the protein encoded by human or S.pombe rad9 reveals a BH3-like domain in the N-terminal region that can bind the anti-apoptotic proteins Bcl-2 and Bcl-XL. Furthermore, overexpression of the gene from either organism in human cells can cause apoptosis in a BH3 domain-dependent manner. Both S.pombe and human versions of the protein can bind two other checkpoint control proteins, Hus1p (HUS1p) and Rad1p (RAD1p). Human RAD9 protein binds HUS1 and RAD1 proteins at its C-terminal region, suggesting that RAD9 has at least two functional domains, one involved in apoptosis and the other in cell cycle checkpoint control. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p, is hyperphosphorylated by Mec1p and Tel1p, and is a potential Cdc28p substrate.
- 基因ID
- 851803
- NCBI登录号
- NP_010503
- UniProt
- P14737
- 途径
- Positive Regulation of Response to DNA Damage Stimulus
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