PPARA 抗体 (N-Term)
(3 references)
Quick Overview for PPARA 抗体 (N-Term) (ABIN105798)
抗原
See all PPARA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-401-421
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Supplier
- Rockland
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原理
- PPAR alpha Antibody
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交叉反应 （详细）
- Anti-PPAR alpha Antibody is directed against mouse PPAR alpha protein.
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产品特性
- Synonyms: rabbit anti-Ppar alpha antibody, Pparα, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Ppar-a, Nr1c1, Ppar
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 301-400) antibody
PPARA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 61-170) antibodyPPARA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-120) antibodyPPARA 适用: 人, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5D10C3 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-468) antibodyPPARA 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 1331CT894-186-143 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyKO Validated PPARA 适用: 人 WB, IP, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-120) antibodyPPARA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5D10E10 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (C-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, Pig, 山羊, 豚鼠, 兔, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (pSer12) antibodyPPARA 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 山羊, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:100-1:300
Application Note: Anti-PPAR alpha Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:75,000 - 1:125,000
IF Microscopy Dilution: 1-5 μg/mL
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." in: Leukemia, Vol. 24, Issue 8, pp. 1476-86, (2010) (PubMed).
: "Medicine. PPARs as therapeutic targets: reverse cardiology?" in: Science (New York, N.Y.), Vol. 302, Issue 5644, pp. 406-7, (2003) (PubMed).
: "Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators." in: The Journal of steroid biochemistry and molecular biology, Vol. 51, Issue 3-4, pp. 157-66, (1995) (PubMed).
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: "Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." in: Leukemia, Vol. 24, Issue 8, pp. 1476-86, (2010) (PubMed).
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- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
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别名
- Ppara
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背景
- Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements. Anti-Ppar Antibody is useful for research interested in transcription and metabolic pathways.
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基因ID
- 19013, 31543500
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UniProt
- P23204
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途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
抗原
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