PLG 抗体 (Biotin)
Quick Overview for PLG 抗体 (Biotin) (ABIN105703)
抗原
See all PLG 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-106-208-0100
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Supplier
- Rockland
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原理
- Plasminogen Antibody Biotin Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma].
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产品特性
- Synonyms: goat anti-Plasminogen Antibody, biotin Conjugated goat anti-Plasminogen Antibody, Angiostatin antibody, Microplasmin antibody, Plasmin antibody, Plasmin heavy chain A antibody, Plasmin light chain B antibody, PLG antibody
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纯化方法
- Anti-Plasminogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Plasminogen [Human Plasma]
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Application Note: Anti-Plasminogen antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 μg of Plasminogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PLG (Plasminogen (PLG))
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别名
- PLG
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背景
- Background: Anti-Plasminogen antibody recognizes plasminogen, a precursor of plasmin. Plasmin is a serine protease that degrades fibrin clots and involved in embryonic development, tissue remodeling, tumor invasion, and inflammation. In addition to fibrin, Plasmin can also cleave fibronectin, thrombospondin, laminin, and von Willebrand factor. Plasmin can activate other zymogens such as tissue plasminogen activator, urokinase plasminogen activator, kallikrein, and factor XII. Deficiency in plasminogen increases susceptibility to thrombosis.
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基因ID
- 5340
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UniProt
- P00747
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途径
- Complement System, Lipid Metabolism
抗原
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