PPY 抗体
Quick Overview for PPY 抗体 (ABIN105318)
抗原
See all PPY 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 214-a01-118
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Supplier
- Rockland
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原理
- Pancreatic Islet Lysate Antibody
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum.
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产品特性
- Synonyms: IgG fraction of Anti-pancreatic islet lysate, Dog Anti-Pancreatic lysate (porcine pancreas), Anti-pancreatic lysate produced in dog, dog anti-Pancreatic Islet Lysate Antibody, canine anti-Pancreatic Islet Lysate Antibody
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
- Optional[Flow Cytometry Dilution]: This product has been assayed against 1.0 μg of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PPY (Pancreatic Polypeptide (PPY))
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别名
- PPY
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背景
- Background: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. It may be used as a marker of the viability of xenotransplanted fetal pancreatic tissue in the immediate post-transplant period.
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UniProt
- P01300
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途径
- Feeding Behaviour
抗原
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