VIPR2 抗体 (Internal Region)
Quick Overview for VIPR2 抗体 (Internal Region) (ABIN1049457)
抗原
See all VIPR2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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品牌
- IHC-plus™
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特异性
- Human VIPR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
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预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%) Rat, Pig (94%) Mouse, Dog, Rabbit, Opossum (88%) Elephant (81%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic 16 amino acid peptide from internal region of human VIPR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%), Rat, Pig (94%), Mouse, Dog, Rabbit, Opossum (88%), Elephant (81%).
Type of Immunogen: Synthetic peptide
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anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (AA 81-170) antibody
VIPR2 适用: 人, 大鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (Internal Region) antibodyVIPR2 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (AA 24-126) antibodyVIPR2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (AA 24-126) antibodyVIPR2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (AA 25-37), (Extracellular) antibodyVIPR2 适用: 人 WB, IF, FACS, IC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) antibodyVIPR2 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (AA 24-126) antibodyVIPR2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (C-Term) antibodyVIPR2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 2 (VIPR2) (AA 95-123), (N-Term) antibodyVIPR2 适用: 人 WB 宿主: 兔 Polyclonal RB36748 unconjugated
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应用备注
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
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Target Species of Antibody: Human
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实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, less than 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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有效期
- 12 months
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- VIPR2 (Vasoactive Intestinal Peptide Receptor 2 (VIPR2))
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别名
- VPAC2 / VIPR2
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背景
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Name/Gene ID: VIPR2
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR
Synonyms: VIPR2, C16DUPq36.3, DUP7q36.3, PACAP-R-3, Pvr3, Vip type-2 receptor, Vip-r2, Vip2 receptor, PACAP type III receptor, Pacap type-3, PACAP-R3, PACAPR-3, VIP and PACAP receptor 2, VIP-R-2, VIP2R, VPAC2, VPCAP2R, PACAP3, Vip2, VPAC2R -
基因ID
- 7434
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途径
- cAMP Metabolic Process
抗原
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