EPH Receptor B2 抗体 (Internal Region)
-
- 抗原 See all EPH Receptor B2 (EPHB2) 抗体
- EPH Receptor B2 (EPHB2)
-
抗原表位
- Internal Region
- 适用
- 人, 小鼠, 大鼠, 猴, 兔, 斑马鱼
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This EPH Receptor B2 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human EPHB2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%) Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%) Xenopus, Seabass (87%) Stickleback (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 15 amino acid peptide from internal region of human EPHB2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%), Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%), Xenopus, Seabass (87%), Stickleback (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product EPHB2 Primary Antibody
-
anti-EPH Receptor B2 (EPHB2) antibody
EPHB2 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 48CT12-6-4 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 551-650) antibodyEPHB2 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 226-325) antibodyEPHB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 17-200) antibodyEPHB2 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 2D12C6 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 1021-1050), (C-Term) antibodyEPHB2 适用: 人 WB, IHC (p), IF 宿主: 兔 Polyclonal RB01652 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 261-465) antibodyEPHB2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (C-Term) antibodyEPHB2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (pTyr298) antibodyEPHB2 适用: 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (pTyr317) antibodyEPHB2 适用: 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (pTyr331) antibodyEPHB2 适用: 小鸡 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (7 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- EPH Receptor B2 (EPHB2)
- 别名
- EPHB2 / EPH Receptor B2 (EPHB2 产品)
- 别名
- CAPB antibody, DRT antibody, EK5 antibody, EPHT3 antibody, ERK antibody, Hek5 antibody, PCBC antibody, Tyro5 antibody, ERK2 antibody, ERT1 antibody, MAPK2 antibody, P42MAPK antibody, PRKM1 antibody, PRKM2 antibody, p38 antibody, p40 antibody, p41 antibody, p41mapk antibody, RGD1564232 antibody, Cek5 antibody, Drt antibody, ETECK antibody, Erk antibody, Nuk antibody, Prkm5 antibody, Qek5 antibody, Sek3 antibody, cek5 antibody, ephb2 antibody, xEphB2 antibody, si:ch73-160e9.1 antibody, tpx2 antibody, wu:fb09e10 antibody, wu:fc64a03 antibody, wu:fi18g12 antibody, zgc:112296 antibody, EPH receptor B2 antibody, mitogen-activated protein kinase 1 antibody, Eph receptor B2 antibody, EPH receptor B2 L homeolog antibody, eph receptor B2a antibody, eph receptor B2b antibody, EPHB2 antibody, MAPK1 antibody, Ephb2 antibody, ephb2.L antibody, ephb2a antibody, ephb2b antibody
- 背景
-
Name/Gene ID: EPHB2
Subfamily: Ephrin Receptor
Family: Protein Kinase
Synonyms: EPHB2, CAPB, Cek5, EK5, ETECK, EPH tyrosine kinase 3, DRT, ELK-related tyrosine kinase, Hek5, EPH-like kinase 5, EPHT3, Nuk, PCBC, Tyrosine-protein kinase TYRO5, Protein-tyrosine kinase HEK5, Tyro5, EPH receptor B2, Ephrin type-B receptor 2, EPTH3 - 基因ID
- 2048
- 途径
- RTK signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
-
