DRD5 抗体 (Cytoplasmic Domain)
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- 抗原 See all DRD5 抗体
- DRD5 (Dopamine Receptor D5 (DRD5))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DRD5 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DRD5 / Dopamine Receptor D5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog (100%) Marmoset, Panda, Horse (94%) Mouse, Bovine, Hamster, Elephant, Pig (88%) Rat, Rabbit (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human DRD5 / Dopamine Receptor D5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog (100%), Marmoset, Panda, Horse (94%), Mouse, Bovine, Hamster, Elephant, Pig (88%), Rat, Rabbit (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DRD5 Primary Antibody
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anti-Dopamine Receptor D5 (DRD5) (AA 361-477) antibody
DRD5 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D5 (DRD5) (Internal Region) antibodyVerified DRD5 适用: 人 ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Dopamine Receptor D5 (DRD5) (AA 323-477) antibodyDRD5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D5 (DRD5) (2nd Extracellular Loop), (AA 199-211) antibodyDRD5 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D5 (DRD5) (AA 251-350) antibodyDRD5 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D5 (DRD5) (N-Term) antibodyDRD5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D5 (DRD5) (AA 421-456), (C-Term) antibodyDRD5 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB52763 unconjugated
anti-Dopamine Receptor D5 (DRD5) (AA 19-56), (N-Term) antibodyDRD5 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52770 unconjugated
anti-Dopamine Receptor D5 (DRD5) (C-Term) antibodyDRD5 适用: 大鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal SG4-D1b unconjugated
anti-Dopamine Receptor D5 (DRD5) (Cytoplasmic Domain) antibodyDRD5 适用: 人, 犬, Gorilla IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DRD5 (Dopamine Receptor D5 (DRD5))
- 别名
- DRD5 / Dopamine Receptor D5 (DRD5 产品)
- 别名
- DRD5 antibody, DBDR antibody, DRD1B antibody, DRD1L2 antibody, D1B antibody, D5R antibody, Drd-5 antibody, Drd1b antibody, Gpcr1 antibody, dopamine receptor D5 antibody, DRD5 antibody, Drd5 antibody
- 背景
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Name/Gene ID: DRD5
Subfamily: Dopamine
Family: GPCR
Synonyms: DRD5, D(5) dopamine receptor, D1beta dopamine receptor, D5 dopamine receptor, Dopamine 5 receptor, Dopamine receptor D5, DRD1B, D(1B) dopamine receptor, D1b dopamine receptor, D1b receptor, DBDR, Dopamine receptor 5, DRD1L2, Hd5dr, Dopamine d1b receptor, Dopamine D5 receptor, Dopamine receptor D1B - 基因ID
- 1816
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity
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