DKK1 抗体 (Internal Region)
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- 抗原 See all DKK1 抗体
- DKK1 (Dickkopf Homolog 1 (DKK1))
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抗原表位
- Internal Region
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适用
- 人, 马, Pig, 猴, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DKK1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DKK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Bat, Horse, Pig (100%) Galago, Sheep, Goat, Elephant, Bovine, Rabbit, Guinea pig (94%) Hamster, Panda, Dog (88%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from internal region of human DKK1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Bat, Horse, Pig (100%), Galago, Sheep, Goat, Elephant, Bovine, Rabbit, Guinea pig (94%), Hamster, Panda, Dog (88%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DKK1 Primary Antibody
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anti-Dickkopf Homolog 1 (DKK1) (AA 1-266) antibody
DKK1 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal RB52126 unconjugated
anti-Dickkopf Homolog 1 (DKK1) (AA 1-266) antibodyDKK1 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 2A5 unconjugated
anti-Dickkopf Homolog 1 (DKK1) (AA 33-266) antibodyDKK1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Dickkopf Homolog 1 (DKK1) (AA 81-180) antibodyDKK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4C10 unconjugated
anti-Dickkopf Homolog 1 (DKK1) (AA 201-266) antibodyDKK1 适用: 人, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 1 (DKK1) (AA 36-260) antibodyDKK1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 1 (DKK1) (AA 33-266) antibodyDKK1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 1 (DKK1) (AA 33-266) antibodyDKK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 1 (DKK1) (Cytoplasmic Domain) antibodyDKK1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 1 (DKK1) (Internal Region) antibodyDKK1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 别名
- DKK1 (DKK1 产品)
- 别名
- DKK-1 antibody, SK antibody, AI414495 antibody, SGY antibody, SGY-1 antibody, SGY1 antibody, Soggy antibody, mSgy antibody, dkk1 antibody, dkk antibody, dkk-1 antibody, xdkk1 antibody, Xdkk-1 antibody, Dickkopf1 antibody, dickkopf-1 antibody, mdkk-1 antibody, dkk1-A antibody, dickkopf WNT signaling pathway inhibitor 1 antibody, dickkopf like acrosomal protein 1 antibody, dickkopf-related protein 1 antibody, dickkopf-like 1 antibody, dickkopf WNT signaling pathway inhibitor 1b antibody, dickkopf protein antibody, dickkopf WNT signaling pathway inhibitor 1 S homeolog antibody, DKK1 antibody, Dkk1 antibody, DKKL1 antibody, LOC100228789 antibody, Dkkl1 antibody, dkk1b antibody, dkk1 antibody, dickkopf antibody, dkk1.S antibody
- 背景
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Name/Gene ID: DKK1
Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK - 基因ID
- 22943
- 途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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