NFYA 抗体 (N-Term)
(4 references)
Quick Overview for NFYA 抗体 (N-Term) (ABIN104769)
抗原
See all NFYA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 200-401-100
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Supplier
- Rockland
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原理
- NF-Y Antibody
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交叉反应 （详细）
- A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.
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产品特性
- Synonyms: rabbit anti-NF-Y Antibody, Nf-Y-A subunit, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A
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纯化方法
- Anti-NF-Y (a) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibody
NFYA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Pig, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig WB, IHC, IF, IP, IC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-NF-Y antibody is tested for western blotting and suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Infinity: An In-Silico Tool for Genome-Wide Prediction of Specific DNA Matrices in miRNA Genomic Loci." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153658, (2016) (PubMed).
: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, (2008) (PubMed).
: "Repression of the antiapoptotic molecule galectin-3 by homeodomain-interacting protein kinase 2-activated p53 is required for p53-induced apoptosis." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4746-57, (2006) (PubMed).
: "The long terminal repeat (LTR) of ERV-9 human endogenous retrovirus binds to NF-Y in the assembly of an active LTR enhancer complex NF-Y/MZF1/GATA-2." in: The Journal of biological chemistry, Vol. 280, Issue 42, pp. 35184-94, (2005) (PubMed).
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: "Infinity: An In-Silico Tool for Genome-Wide Prediction of Specific DNA Matrices in miRNA Genomic Loci." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153658, (2016) (PubMed).
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- NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
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别名
- NFYA
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背景
- Background: Anti-NF-Y antibody detects NF-Y (a subunit) protein. NF-Y is a highly conserved transcription factor that stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. NF-subunit A associates with a tight dimer composed of the NF-YB and NF-YC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. Anti-NF-YA Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research.
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基因ID
- 4800
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UniProt
- P23511
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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